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US 6,194,550 B1Granted
Systematic polypeptide evolution by reverse translation
OwnerLarry Gold, Craig Tuerk +2inventors
InventorsLarry Gold, Craig Tuerk, David Pribnow, Jonathan Drew Smith
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Abstract
A method for preparing polypeptide ligands of target molecules wherein candidate mixtures comprised of ribosome complexes or mRNA•polypeptide copolymers are partitioned relative to their affinity to the target and amplified to create a new candidate mixture enriched in ribosome complexes or mRNA•polypeptide copolymers with an affinity to the target.
Claims9
A method for isolating a nucleic acid.polypeptide copolymer ligand to a target molecule comprising:
- (a) synthesizing a nucleic acid mixture comprising mRNA, wherein said mRNA comprises translatable and non-translatable regions;
- (b) synthesizing a mixture of nucleic acid.polypeptide copolymers, each copolymer comprising said mRNA and a polypeptide encoded by its associated mRNA, and not containing a ribosome;
- and
- (c) partitioning the nucleic acid.polypeptide copolymers with respect to affinity of the copolymers to a desired target molecule.
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