DNA encoding a hybrid heterodimeric protein
AssigneeApplied Research Systems ARS Holding N.V.
InventorsRobert K. Campbell, Bradford A. Jameson, Scott C. Chappel
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Abstract
A hybrid protein includes two coexpressed amino acid sequences forming a dimer. Each sequence contains the binding portion of a receptor, such as TBP1 or TBP2, or a ligand, such as IL-6, IFN-βand TPO, linked to a subunit of a heterodimeric proteinaceous hormone, such as hCG. Each coexpressed sequence contains a corresponding hormone subunit so as to form a heterodimer upon expression. Corresponding DNA molecules, expression vectors and host cells are also disclosed as are pharmaceutical compositions and a method of producing such proteins.
Claims4
An isolated DNA molecule encoding a hybrid protein, wherein said hybrid protein comprises two different coexpressed amino acid sequences forming a heterodimer, each comprising:
- (a) at least one amino acid sequence selected from the group consisting of a chain of a homomeric receptor, a chain of a heteromeric receptor, a ligand other than a gonadotropin, a fragment of said chain of said homomeric receptor, said chain of said heteromeric receptor or said ligand, wherein said ligand or fragment thereof retains ligand-receptor binding capability and said chain of said homomeric receptor or fragment thereof and said chain of said heteromeric receptor or fragment thereof retain ligand-receptor binding capability either alone or in association with a homologous or heterologous chain of said receptor;
- and
- (b) a subunit of a heterodimeric proteinaceous hormone, or a fragment thereof which retains the ability of the subunit to form a heterodimer with other subunits thereof;
- wherein sequences (a) and (b) are joined either directly or through a peptide linker, and in which the sequences (b) in each of said two different coexpressed sequences aggregate with each other to dimerize and form a heterodimer.
Dependencies are inferred from the claim text.