Water-soluble rhodamine dyes and conjugates thereof
AssigneePE Corporation
InventorsLinda G. Lee, Ronald J. Graham, William E. Werner, Elana Swartzman, Lily Lu
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Abstract
The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
Claims23
A rhodamine dye or a salt thereof, comprising a rhodamine-type parent xanthene ring having attached to the xanthene C9 carbon a phenyl group that is further substituted with an ortho carboxy or ortho sulfonate group or a salt thereof, one to three substituted or unsubstituted aminopyridinium groups and a substituted or unsubstituted alkylthio, or arylthio group, said rhodamine dye optionally including one or more linking moieties selected from the group consisting of an azido, a monosubstituted primary amine, a disubstituted secondary amine, a thiol, an hydroxyl, a halide, an epoxide, an N-hydroxysuccinimidyl ester, a carboxyl, and an activated ester.
Dependencies are inferred from the claim text.