Compositions and methods relating to DNA mismatch repair genes
AssigneesDana-Farber Cancer Institute, Inc., Oregon Health Sciences University
InventorsRobert M. Liskay, C. Eric Bronner, Sean M. Baker, Roni J. Bollag, Richard D. Kolodner
Life of this patent
Estimating the term…
Abstract
Genomic sequences of human mismatch repair genes are described, as are methods of detecting mutations and/or polymorphisms in those genes. Also described are methods of diagnosing cancer susceptibility in a subject, and methods of identifying and classifying mismatch-repair-defective tumors. In particular, sequences and methods relating to human mutL homologs, hMLH1 and hPMS1 genes are provided.
Claims79
An isolated nucleic acid molecule including a segment having a sequence selected from the group consisting of SEQ ID NOS:
- 6-24.
An isolated nucleic acid molecule including a segment having a sequence selected from the group consisting of SEQ ID NOS:
- 25-43.
- 3.A unique fragment of the nucleic acid molecule of claim 1 or claim 2.
An isolated polynucleotide including a unique segment of a human mutL homolog gene, wherein the mutL homolog gene is hMLH1, or hPMS1.
An isolated human nucleic acid molecule comprising a segment of at least 13 nucleotides the same as any 13 nucleotide sequence in any one of SEQ ID NOS:
- 6-24.
An isolated polynucleotide comprising a segment of at least 13 nucleotides the same as any 13 nucleotide sequence in SEQ ID NO:
- 145.
An isolated polynucleotide comprising a segment of at least 13 nucleotides the same as any 13 nucleotide sequence in any one of SEQ ID NOS:
- 7-24.
An isolated polynucleotide comprising a sequence of at least 13 nucleotides the same as any 13 nucleotide sequence in any one of SEQ ID NOS:
- 26-43.
A composition comprising isolated nucleic acid molecules containing a human sequence encoding hMLH1, wherein said hMLH1 sequence is selected from the group consisting of:
- (a) SEQ ID NOS:
- 6-24, (b) nucleic acid sequences complementary to (a), and (c) nucleic acid sequences containing a segment of at least 13 nucleotides the same as any 13 nucleotide sequence in (a) or (b).
An isolated nucleotide sequence encoding an hMLH1 protein having an amino acid sequence as set forth in SEQ ID NO:
- 5.
An isolated polynucleotide comprising a segment having the same nucleotide sequence as shown in SEQ ID NO:
- 4 between nucleotide numbers 135-312.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 25.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 26.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 27.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 28.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 29.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 30.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 31.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 32.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 33.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 34.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 35.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 36.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 37.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 38.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 39.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 40.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 41.
An isolated polynucleotide comprising a segment having the nucleotide sequence of SEQ ID NO:
- 42.
Copies of a DNA sequence amplified from DNA or RNA, each copy comprising a segment of at least 13 nucleotides of hPMS1 as shown in SEQ ID NO:
- 132.
An isolated human gene comprising
- exons having a combined nucleotide sequence as shown in SEQ ID NO:
- 4, and introns intervening the exons.
Isolated DNA comprising a first strand containing a first segment of at least 13 nucleotides of hPMS1 cDNA as shown in SEQ ID NO:
- 132, and a second strand containing a second segment which is complementary to at least 13 nucleotides of hPMS1 cDNA as shown in SEQ ID NO:
- 132, wherein the sequences of the segments can be used to design a pair of oligonucleotide primers for amplifying specifically at least a portion of hPMS1.
An isolated human nucleic acid sequence comprising a segment of hPMS1 as shown in the first 500 bases listed in SEQ ID NO:
- 132.
An isolated human nucleic acid sequence comprising a segment of hPMS1 including bases 501 through 1000 as listed in SEQ ID NO:
- 132.
An isolated human nucleic acid sequence comprising a segment of hPMS1 including bases 1001 through 1500 as listed in SEQ ID NO:
- 132.
An isolated human nucleic acid sequence comprising a segment of hPMS1 including bases 1501 through 2000 as listed in SEQ ID NO:
- 132.
An isolated human nucleic acid sequence comprising a segment of hPMS1 including bases 2001 through 2687 as listed in SEQ ID NO:
- 132.
An isolated polynucleotide comprising a segment having a sequence as shown in SEQ ID NOS:
- 146 and 147.
An isolated polynucleotide comprising a first segment of at least 13 nucleotides the same as any 13 nucleotide sequence in SEQ ID NOS:
- 146 and 147.
An isolated polynucleotide comprising a segment having a sequence as shown in SEQ ID NO:
- 149 or a unique fragment thereof.
An isolated polynucleotide comprising a first segment of at least 13 nucleotides the same as any 13 nucleotide sequence in SEQ ID NO:
- 149.
An isolated human polynucleotide comprising a sequence of at least 13 nucleotides the same as any 13 nucleotide sequence in SEQ ID NO:
- 132, wherein the polynucleotide contains at least one mutation that increases a person's risk of developing cancer.
An isolated human polynucleotide comprising a sequence of at least 13 nucleotides the same as any 13 nucleotide sequence in SEQ ID NO:
- 4, wherein the polynucleotide contains at least one mutation that increases a person's risk of developing cancer.
An isolated human polynucleotide comprising a sequence of at least 13 nucleotides the same as any 13 nucleotide sequence in SEQ ID NO:
- 148, wherein the polynucleotide contains at least one mutation that increases a person's risk of developing cancer.
An isolated segment of a human gene, comprising an intron portion and an exon portion, the exon portion corresponding sequentially to a unique fragment of HMLH1 cDNA as shown in SEQ ID NO:
- 145.
Dependencies are inferred from the claim text.