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US 6,191,267 B1Granted
Cloning and producing the N.BstNBI nicking endonuclease
AssigneeNew England Biolabs, Inc.
InventorsHuimin Kong, Lauren S. Higgins, Michael Dalton, Rebecca B. Kucera, Ira Schildkraut
Life of this patent
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Abstract
The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI in non-thio strand displacement amplification, is disclosed also.
Claims5
Isolated DNA coding for the N.BstNBI restriction endonuclease, wherein the isolated DNA is obtainable from ATCC Accession No. PTA-1925.
A vector comprising SEQ ID NO:2.
Dependencies are inferred from the claim text.