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US 6,191,258 B1Granted
Purified palindromic element binding factor
AssigneeThe Salk Institute for Biological Studies
InventorsChristopher J. Lamb, Peter Doerner, Goetz Laible
Life of this patent
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Abstract
A novel transcription enhancer element, (AATT)n, that increases the activity of cis-elements in heterologous promoter constructs without altering their intrinsic specificity is provided. Also provided is a transcription factor, PABF, which specifically binds to the (AATT) repeat element and methods of use therefor.
Claims3
A substantially purified palindromic element binding factor (PABF) polypeptide, characterized as:
- a) having a molecular weight of approximately 67 kD, as determined by SDS-PAGE;
- b) binding to an (AATT)n repeat element, where n≧2;
- and
- c) having an H1 histone domain, a glutamine rich domain, and an HMG/Y domain.
1 more element
A substantially purified palindromic element binding factor (PABF) polypeptide comprising the amino acid sequence as set forth in SEQ ID NO:3.
Dependencies are inferred from the claim text.