Natural or recombinant DNA binding proteins as carriers for gene transfer or gene therapy
AssigneeBaylor College of Medicine
InventorsFred D. Ledley, Jozsef Stankovics
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Abstract
A complex for gene transfer a DNA molecule specifically and non-specifically bound to DNA binding protein. Additionally, it can include a chimeric compound for gene transfer. The chimeric compound has a DNA-binding element and a ligand binding element. The chimeric recombinant DNA can also include a binding protein which has a first element for binding to a receptor, a second element for binding to DNA, a third element for destabilizing endosomes and a fourth element for directing the traffic in a protein containing complex in the nucleus of a cell. The complex will be used for the treatment of a variety of diseases.
Claims51
A chimeric protein produced by a recombinant DNA technique from two domains of separate native proteins comprising:
- (a) a DNA-binding element selected from the group consisting of histone, nuclear hormone receptor, and transacting regulatory element, and
- (b) a ligand-binding element which binds to one or more specific receptors on the surface of target cells and is selected from the group consisting of protein hormone, glycoprotein hormone, immunoglobulin, serum protein, vitamin binding protein, transcobalamin I, transcobalamin II, R binder, intrinsic factor, cytokine, neuropeptide, and cell adhesion molecule.
A complex for gene transfer comprising:
- (a) a chimeric protein produced by a recombinant DNA technique from two domains of separate native proteins comprising a ligand binding element which binds to one or more specific receptors on the surface of target cells and a DNA-binding element selected from the group consisting of histone, nuclear hormone receptor, and transacting regulatory element;
- and
- (b) DNA bound nonspecifically to said DNA-binding element of said chimeric protein;
- wherein
- said ligand-binding element is selected from the group consisting of protein hormone, glycoprotein hormone, immunoglobulin, serum protein, vitamin binding protein, transcobalamin I, transcobalamin II, R binder, intrinsic factor, cytokine, neuropeptide, and cell adhesion molecule.
A carrier for gene transfer comprising a DNA-binding element and a ligand binding element, wherein the improvement consists of the carrier comprising a chimeric protein produced by a recombinant DNA technique from two domains of separate native proteins, wherein:
- (a) said DNA-binding element is selected from the group consisting of histone, nuclear hormone receptor, and transacting regulatory element;
- and
- (b) said ligand-binding element binds to one or more receptors or the surface of target cells and is selected from the group consisting of protein hormone, glycoprotein hormone, immunoglobulin, serum protein, vitamin binding protein, transcobalamin I, transcobalamin II, R binder, intrinsic factor, cytokine, neuropeptide, and cell adhesion molecule.
Dependencies are inferred from the claim text.