Expression vector for the regulatable expression of foreign genes in prokaryotes
AssigneeRoche Diagnostics GmbH
InventorsGünther Schumacher, Michael Jarsch, Winfried Boos
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Abstract
The invention provides an expression vector useful in expressing foreign genes in procaryotes. The vector consists of the promotor/operator region and initiation point of translation of the mg1 operon. This combination is referred to as a regulation sequence. In order to prepare this, desired portions of the mg1 operon are cut from the genome of a cell which can utilize galactose via appropriate restriction endonucleases. This is then inserted into a DNA vector.
Claims14
An expression vector comprising a regulation sequence consisting essentially of a promoter/operator region of an E. coli or S. tymphimurium mg1B operon up to but not including the ATG codon which is the initiation point of translation, wherein said expression vector does not include any sequences which code for mature mg1B protein.
Isolated nucleic acid molecule consisting of the E. coli or S. tymphimurium mg1 promoter/operator region up to, but not including the ATG codon which is the initiation point of translation of an mg1 operon.
Isolated nucleic acid molecule consisting of nucleotides 1 to 704 of FIG. 1.
Isolated nucleic acid molecule consisting of nucleotides 1-773 of FIG. 1.
Plasmid M13mg1506.
Plasmid M13mg1EcoK.
Dependencies are inferred from the claim text.