Enzyme with protease activity
OwnerHenrik Dalbøge, Stephan Christgau +5inventors
InventorsHenrik Dalbøge, Stephan Christgau, Lene Nonboe Andersen, Lene Venke Kofod, Markus Sakari Kauppinen, Jack Bech Nielsen, Claus Dambmann
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Abstract
An isolated and purified enzyme exhibiting protease activity at a pH of 4-7 which exhibits protease in 5% hydrogen peroxide and which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO: 1 or 2. Methods are described for using the protease compositions in reducing vescosity, cleaning contact lenses, baking, and preparing animal feed.
Claims4
A method for cleaning a contact lens comprising treating said contact lens with an effective amount of an isolated and purified enzyme exhibiting protease activity at a pH of 4-7 which exhibits protease activity in 5% hydrogen peroxide and which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO. 1 or 2 under the following conditions:
- hybridizing in 5×SSC, 5×Denhardt's solution, 0.5% SDS and 100 ug/ml salmon sperm DNA for 16 hrs. at about 65° C., followed by washes in 5×SSC, 2×SSC, 0.5% SDS, 0.2×SSC, 0.5% SDS and 5×SSC at 65° C.
Dependencies are inferred from the claim text.