Deformylation of f-met peptides in bacterial expression system
AssigneeMonsanto Company
InventorGregg Bogosian
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Abstract
A method for producing recombinant peptides and proteins having a diminished retention of N-formyl-methionine by coexpressing a peptide deformylase enzyme is disclosed. Also disclosed are substantially deformylated protein compositions and transformed bacterial cells and DNA vectors useful for acheiving such deformylation.
Claims5
A transformed E. coli host cell comprising a DNA sequence encoding an E. Coli peptide deformylase enzyme operably linked to a promoter operable in E. coli, which transformed E. coli host cell is able to produce more peptide deformylase enzyme than an untransformed E. coli host cell.
A cell extract comprising a recombinant protein produced from bacterial host cells which is 96 to 100% deformylated.
A cell extract comprising a recombinant protein produced from bacterial host cells which is 98 to 100% deformylated.
A cell extract comprising a bovine somatotropin produced from bacterial host cells which is 96 to 100% deformylated.
A cell extract comprising a bovine somatotropin produced from bacterial host cells which is 98 to 100% deformylated.
Dependencies are inferred from the claim text.