Subtilase variants
AssigneeNovo Nordisk A/S
InventorsLaurens Nicolaas Sierkstra, Jan Klugkist, Peter Markvardsen, Claus von der Osten, Peter Bauditz
Life of this patent
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Abstract
The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.
Claims51
A modified subtilase comprising at least two substitutions of the naturally-occurring amino acid residue with a more hydrophobic amino acid residue at positions selected from the group consisting of:
- 129, 131, 136, 159, 164, 165, 167, 170, 171, 194 and 195
- wherein each position corresponds to a position of the amino acid sequence of subtilisin BPN′.
A modified subtilase comprising a substitution selected from the group consisting of:
- (a) a substitution of the amino acid residue at position 129 with Val, Ile, Leu, Met, Phe, Pro or Trp,
- (b) a substitution of the amino acid residue at position 131 with Val, Ile, Leu, Met, Phe, Pro or Trp,
- (c) a substitution of the amino acid residue at position 136 with Val, Ile, Leu, Met, Phe, Pro or Trp,
- (d) a substitution of the amino acid residue at position 159 with Val, Ile, Leu, Met, Phe, Pro or Trp,
- (e) a substitution of the amino acid residue at position 164 with Val, Ile, Leu, Met, Phe, Pro or Trp,
- (f) a substitution of the amino acid residue at position 165 with Val, Ile, Leu, Met, Phe, Pro or Trp,
- (g) a substitution of the amino acid residue at position 167 with Ile or Leu,
- (h) a substitution of the amino acid residue at position 170 with Leu, Phe, Pro or Trp,
- (i) a substitution of the amino acid residue at position 171 with Val, Ile or Leu,
- (j) a substitution of the amino acid residue at position 194 with Leu, and
- (k) a substitution of the amino acid residue at position 195 with Val, Ile, Leu, Met, Phe, Pro or Trp,
- wherein each position corresponds to a position of the amino acid sequence of subtilisin BPN′.
Dependencies are inferred from the claim text.