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US 6,190,886 B1Granted
Trimerizing polypeptides, their manufacture and use
OwnerHans-Jürgen Hoppe, Kenneth B. Reidinventors
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OwnerHans-Jürgen Hoppe, Kenneth B. Reidinventors
Estimating the term…
Polypeptides comprising a collectin neck region, or variant or derivative thereof or amino acid sequence having the same or a similar amino acid pattern and/or hydrophobicity profile, are able to trimerize. Such polypeptides may comprise additional amino acids which may include heterologous amino acids, for example, forming a protein domain or derived from an immunoglobulin or comprising an amino acid which may be derivatized for attachment of a non-peptide moiety such as oligosaccharide, and may form homotrimers or heterotrimers. Heterotrimerization may be promoted by gentle heating, e.g. to about 50° C., then cooling to room temperature. One use for the polypeptides is in seeding collagen formation. Nucleic acid encoding the polypeptides and methods of their production are provided.
A non-naturally occurring polypeptide which comprises a first sequence of amino acids which is a neck-region of a collectin, fused to one or more heterologous amino acids, and which forms a trimer.
A non-naturally occurring polypeptide that forms a trimer and that comprises a first sequence of amino acids that is a neck-region of a collectin that contains at least one substitution of an amino acid residue, wherein said substitution is determined by amino acid sequence alignment of said neck-region with a neck-region of a different collectin to be present at the corresponding position in said neck-region of said different collectin, wherein said substitution does not abolish trimerization, fused to one or more heterologous amino acids.
A method of forming a collagenous triple helix comprising providing non-naturally occurring polypeptides, each polypeptide comprising a series of collagenous triplets N-terminal to a first sequence of amino acids which is a neck-region of a collectin and bringing into contact said polypeptides to form trimers.
A method of forming a collagenous triple helix comprising providing non-naturally occurring polypeptides, each polypeptide comprising a series of collagenous triplets N-terminal to a first sequence of amino acids that is a neck-region of a collectin that contains at least one substitution of an amino acid residue, wherein said substitution is determined by amino acid sequence alignment of said neck-region with a neck-region of a different collectin to be present at the corresponding position in said neck-region of said different collectin, wherein said substitution does not abolish trimerization, and bringing into contact said polypeptides to form trimers.
A polypeptide consisting of a neck-region of a collectin, wherein said polypeptide forms a trimer.
A polypeptide consisting of the neck-region amino acid sequence shown in SEQ ID NO:1, wherein said polypeptide forms a trimer.
A polypeptide consisting of a sequence of amino acids that is a neck-region of a collectin that contains at least one substitution of an amino acid residue, wherein said substitution is determined by amino acid sequence alignment of said neck-region with a neck-region of a different collectin to be present at the corresponding position in said neck-region of said different collectin, wherein said substitution does not abolish trimerization, wherein said polypeptide forms a trimer.
Dependencies are inferred from the claim text.