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US 6,190,879 B1Granted
Microbial transglutaminases, their production and use
AssigneeNovo Nordisk A/S
InventorsLisbeth Bech, Iben Angelica Nørrevang, Torben Halkier, Grethe Rasmussen, Thomas Schäfer, Jens Tønne Andersen
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Abstract
A method for identifying a transglutaminase-producing microorganism based on a selective assay is disclosed.
Claims8
A method for identifying a transglutaminase-producing microorganism, said method comprising:
- a) cultivating a microorganism in a liquid culture to obtain a culture broth;
- b) measuring the ability of the broth to catalyze incorporation of putrescine into casein;
- c) measuring the ability of the broth to catalyze conversion of hydroxylamine to hydroxamic acid;
- and
- d) selecting microorganisms wherein the broth (i) catalyzes incorporation of putrescine into casein and (ii) does not catalyze the conversion of hydroxylamine to hydroxamic acid.
2 more elements
Dependencies are inferred from the claim text.