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US 6,190,866 B1Granted
Methods of bacterial gene function determination using peptide nucleic acids
AssigneePeter E. Nielsen
InventorsPeter E. Nielsen, Liam Good
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Abstract
Methods of and compositions for killing or inhibiting the growth of a bacteria are disclosed. Methods of determing bacterial gene functions are also disclosed. The methods comprise the use of peptide nucleic acid that is targeted to mRNA and/or rRNA. In certain embodiments, methods include the use of one or more separate antibiotics.
Claims8
A process for determining the function of a target gene in a bacteria comprising the steps of:
- a) selecting a target gene within said bacteria;
- b) providing a nucleotide sequence of said target gene;
- c) selecting and preparing one or more PNA compounds each having a region that is complementary, in an anti-parallel orientation, to a portion of said nucleotide sequence of said target gene;
- d) determining the activity of said one or more PNA compounds in a selected assay to identify active PNA compounds;
- e) contacting said bacteria with said active PNA compounds;
- and
- f) determining the effect of said one or more PNA compounds on said bacteria.
4 more elements
Dependencies are inferred from the claim text.