HCV genomic sequences for diagnostics and therapeutics
AssigneeChiron Corporation
InventorsTai-An Cha, Eileen Beall, Bruce Irvine, Janice Kolberg, Michael S. Urdea
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Abstract
The present application features nucleic acid, peptide and antibody compositions relating to genotypes of hepatitis C virus and methods of using such compositions for diagnostic and therapeutic purposes.
Claims12
A method of forming a hybridization product with a hepatitis C virus nucleic acid comprising the steps of:
- a. placing an isolated or synthesized non-naturally occurring nucleic acid comprising a nucleotide sequence of fifteen or more contiguous nucleotides fully homologous to or fully complementary to a sequence within a non-HCV-1, hepatitis C viral genome, wherein said sequence is selected from the group consisting of SEQ ID NOs:
- 13, 18, and 24, into conditions in which hybridization conditions can be imposed, wherein said isolated or synthesized nucleic acid forms a hybridization product with said hepatitis C virus nucleic acid, if present, under hybridization conditions;
- and
- b. imposing hybridization conditions to form a hybridization product in the presence of hepatitis C virus nucleic acid.
A method of detecting one or more genotypes of hepatitis C virus comprising the steps of:
- a. placing an isolated or synthesized nucleic non-naturally occurring nucleic acid comprising a nucleotide sequence of fifteen or more contiguous nucleotides fully homologous to or fully complementary to a sequence within a non-HCV-1, hepatitis C viral genome, wherein said sequence is selected from the group consisting of SEQ ID NOs:
- 13, 18, and 24, into conditions in which hybridization conditions can be imposed, wherein said isolated or synthesized nucleic acid forms a hybridization product with said hepatitis C virus nucleic acid, if present, under hybridization conditions;
- and
- b. imposing hybridization conditions to form a hybridization product in the presence of hepatitis C virus nucleic acid;
- and
- c. monitoring the isolated or synthesized nucleic acid for the formation of a hybridization product, which hybridization product is indicative of the presence of the genotype of hepatitis C virus.
A method of forming a hybridization product with a hepatitis C virus nucleic acid comprising the steps of:
- a. placing an isolated or synthesized non-naturally occurring nucleic acid comprising a first nucleotide sequence consisting of from about 15 to about 300 nucleotides wherein at least eight contiguous nucleotides are fully homologous to or fully complementary to a second nucleotide sequence within a non-HCV-1, hepatitis C viral genome, wherein said first nucleotide sequence is selected from the group consisting of SEQ ID NOs:
- 2-6, into conditions in which hybridization conditions can be imposed, wherein said isolated or synthesized nucleic acid forms a hybridization product with said hepatitis C virus nucleic acid, if present, under hybridization conditions;
- and
- b. imposing hybridization conditions to form a hybridization product in the presence of hepatitis C virus nucleic acid.
A method of detecting one or more genotypes of hepatitis C virus comprising the steps of:
- a. placing an isolated or synthesizednucleic non-naturally occurring nucleic acid comprising a first nucleotide sequence consisting of from about 15 to about 300 nucleotides wherein at least eight contiguous nucleotides are fully homologous to or fully complementary to a second nucleotide sequence within a non-HCV-1, hepatitis C viral genome, wherein said first nucleotide sequence is selected from the group consisting of SEQ ID NOs:
- 2-6, into conditions in which hybridization conditions can be imposed, wherein said isolated or synthesized nucleic acid forms a hybridization product with said hepatitis C virus nucleic acid, if present, under hybridization conditions;
- and
- b. imposing hybridization conditions to form a hybridization product in the presence of hepatitis C virus nucleic acid;
- and
- c. monitoring the isolated or synthesized nucleic acid for the formation of a hybridization product, which hybridization product is indicative of the presence of the genotype of hepatitis C virus.
The method of claims 1, 24, 5, or 6 wherein said isolated or synthesized nucleic acid has support means for separating a hybridization product from solution.
Dependencies are inferred from the claim text.