Identification, purification and detection of WSBV baculovirus associated with white spot syndrome
AssigneeNational Science Council
InventorsGuang-Hsiung Kou, Chung-Hsiung Wang, Chu-Fang Lo
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Abstract
This invention relates to the identification, purification and detection of a new infectious viral agent in arthropods, especially shrimps. The virus is named as WSBV (Baculovirus associated with white spot syndrome). Two WSBV genomic DNA libraries were constructed and based upon the sequence of one of the cloned WSBV DNA fragments, a WSBV specific primer set for PCR to detect the WSBV infection in penaeid shrimps has been developed. The results of the present invention provide an effective diagnostic tool for screening WSBV infection in animal host organisms, in particular shrimps, to prevent the further spread of this viral disease.
Claims13
An isolated non-occluded baculovirus associated with white spot syndrome in an arthropod host, the baculovirus being identical to that deposited in the China Center for Type Culture Collection as Accession Number CCTCC-V96001.
An isolated non-occluded baculovirus associated with white spot syndrome in an arthropod host, the baculovirus comprising a genomic DNA molecule comprising the nucleotide sequence of SEQ ID NO:1.
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:1 or the complement thereof.
A nucleic acid segment of a nucleic acid consisting of the sequence of SEQ ID NO:1 or the complement thereof, wherein the segment is 18-25 base pairs in length.
An isolated nucleic acid comprising the nucleotide sequence of SEQ ID NO:2 or the complement thereof.
A nucleic acid segment of a nucleic acid consisting of the sequence of nucleotides 9 to 1455 of SEQ ID NO:1, or the complement thereof, wherein the segment is 18-25 base pairs in length.
Dependencies are inferred from the claim text.