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US 6,190,861 B1Granted
Molecular sequences of swine retroviruses method of using
AssigneeThe General Hospital Corporation
InventorJay A. Fishman
Life of this patent
Estimating the term…
Abstract
Purified nucleic acid which can specifically hybridize with the sequence of swine retroviruses.
Claims93
A method for screening a cell or a tissue for the presence or expression of a swine or miniature swine retrovirus comprising:
- contacting a target nucleic acid from the cell or tissue with a second nucleic acid selected from the group of:
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:1, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:2, or its complement of the same length;
- a sequence which can specifically hybridize to tie sequence of SEQ ID NO:3, or its complement of the same length;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a gag protein, said sense or antisense sequence being selected from the group consisting of gag-encoding nucleotides of SEQ ID NO:1, gag-encoding nucleotides of SEQ ID NO:2, and gag-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2452-4839 or nucleotides 3112-4693 of SEQ ID NO:1, nucleotides 598-2169 of SEQ ID NO:2, or nucleotides 585-2156, of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a pol protein, said sense pol antisense sequence being selected from the group consisting of pol-encoding nucleotides of SEQ ID NO:1, pol-encoding nucleotides of SEQ ID NO:2, and pol-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 4871-8060 of SEQ ID NO:1, nucleotides 2320-4737 of SEQ ID NO:2, or nucleotides 2307-5741 of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes an env protein, said sense or antisense sequence being selected from the group consisting of env-encoding nucleotides of SEQ ID NO:1, env-encoding nucleotides of SEQ ID NO-2, and env-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2-1999 or nucleotides 86-1999 of SEQ ID NO:1, nucleotides 4738-6722 of SEQ ID NO:2, or nucleotides 5620-7533 of SEQ ID NO:3, under conditions in which hybridization can occur, hybridization being indicative of the presence or expression of an endogenous swine or miniature swine retrovirus or retroviral sequence in the cell or tissue.
7 more elements
A method for screening a swine or miniature swine genome for the presence of a porcine retrovirus, comprising:
- contacting the swine or miniature swine genomic DNA with a second nucleic acid selected from the group of:
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:1, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:2, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:3, or its complement of the same length;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a gag protein, said sense or antisense sequence being selected from the group consisting of gag-encoding nucleotides of SEQ ID NO:1, gag-encoding nucleotides of SEQ ID NO:2, and gag-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2452-4839 or nucleotides 3112-4683 of SEQ ID NO:1, nucleotides 598-2169 of SEQ ID NO:2, or nucleotides 585-2156, of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a pol protein, said sense or antisense sequence being selected from the group consisting of pol-encoding nucleotides of SEQ ID NO:1, pol-encoding nucleotides of SEQ ID NO:2, and pol-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 4871-8060 of SEQ ID NO:1, nucleotides 2320-4737 of SEQ ID NO:2, or nucleotides 2307-5741 of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes an env protein, said sense or antisense sequence being selected from the group consisting of env-encoding nucleotides of SEQ ID NO:1, env-encoding nucleotides of SEQ ID NO:2, and env-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2-1999 or nucleotides 86-1999 of SEQ ID NO:1, nucleotides 4738-6722 of SEQ ID NO:2, or nucleotides 5620-7533 of SEQ ID NO:3, under conditions in which the sequences can hybridize, hybridization being indicative of the presence of the endogenous porcine retroviral sequence in the swine or miniature swine genome.
7 more elements
A method of assessing the potential risk associated with the transplantation of a graft from a donor swine or miniature swine into a recipient animal, comprising:
- contacting a target nucleic acid from the donor, recipient, or the graft, with a second nucleic acid selected from the group of:
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:1, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:2, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:3, or its complement of the same length;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a gag protein, said sense or antisense sequence being selected from the group consisting of gag-encoding nucleotides of SEQ ID NO:1, gag-encoding nucleotides of SEQ ID NO:2, and gag-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2452-4839 or nucleotides 3112-4683 of SEQ ID NO:1, nucleotides 598-2169 of SEQ ID NO:2, or nucleotides 585-2156, of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a pol protein, said sense or antisense sequence being selected from the group consisting of pol-encoding nucleotides of SEQ ID NO:1, pot-encoding nucleotides of SEQ ID NO:2, and pol-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 4871-8060 of SEQ ID NO:1, nucleotides 2320-4737 of SEQ ID NO:2, or nucleotides 2307-5741 of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes an env protein, said sense or antisense sequence being selected from the group consisting of env-encoding nucleotides of SEQ ID NO:1, env-encoding nucleotides of SEQ ID NO:2, and env-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2-1999 or nucleotides 86-1999 of SEQ ID NO:1, nucleotides 4738-6722 of SEQ ID NO:2, or nucleotides 5620-7533 of SEQ ID NO:3, under conditions in which the sequences can hybridize, hybridization being indicative of a risk associated with the transplantation.
7 more elements
A method of providing a swine or miniature swine free of an activatable retrovirus insertion at a preselected site, comprising:
- performing a cross between a first swine or miniature swine having a retroviral insertion at the preselected site and a second swine or miniature swine not having a retroviral insertion at a preselected site, and recovering a progeny swine or miniature swine, not having the insertion, wherein the presence or absence of the retroviral insertion is determined by contacting the genome of a swine or miniature swine with a nucleic acid chosen from the group of:
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:1, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:2, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO,3, or its complement of the same length;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a gag protein, said sense or antisense sequence being selected from the group consisting of gag-encoding nucleotides of SEQ ID NO:1, gag-encoding nucleotides of SEQ ID NO:2, and gag-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2452-4939 or nucleotides 31 12-4693 of SEQ ID NO:1, nucleotides 598-2169 of SEQ ID NO:2, or nucleotides 585-2156, of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a pol protein, said sense or antisense sequcnce being selected from the group consisting of pol-encoding nucleotides of SEQ ID NO 1, pol-encoding nucleotides of SEQ ID NO-.2 and pol-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 4971-9860 of SEQ ID NO:1, nucleotides 2320-4737 of SEQ ID NO:2, or nucleotides 2307-5741 of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes an env protein, said sense or antisense sequence being selected from the group consisting of env-encoding nucleotides of SEQ ID NO:1, env-encoding nucleotides of SEQ ID NO:2, and env-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2-1999 or nucleotides 86-1999 of SEQ ID NO;1, nucleotides 4738-6722 of SEQ ID NO:2, or nucleotides 5620-7533 of SEQ ID NO,3.
7 more elements
A method of localizing the origin of a porcine retroviral infection comprising:
- contacting a target nucleic acid from a graft or organ with a second nucleic acid selected from the group of:
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:1, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:2, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:3, or its complement of the same length;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a gag protein, said sense or antisense sequence being selected from the group consisting of gag-encoding nucleotides of SEQ ID NO:1, gag-encoding nucleotides of SEQ ID NO:2, and gag-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2452-4839 or nucleotides 3112-4683 of SEQ ID NO:1, nucleotides 598-2169 of SEQ ID NO:2, or nucleotides 585-2156, of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a pol protein, said sense or antisense sequence being selected from the group consisting of pol-encoding nucleotides of SEQ ID NO:1, pol-encoding nucleotides of SEQ ID NO:2, and pol-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 4871-8060 of SEQ ID NO:1, nucleotides 2320-4737 of SEQ ID NO:2, or nucleotides 2307-5741 of SEQ ID NO,3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes an env protein, said sense or antisense sequence being selected from the group consisting of env-encoding nucleotides of SEQ ID NO:1, env-encoding nucleotides of SEQ ID NO:2, and env-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2-1999 or nucleotides 86-1999 of SEQ ID NO;1, nucleotides 4738-6722 of SEQ ID NO:2, or nucleotides 5620-7533 of SEQ ID NO:3,
- contacting a target nucleic acid from the recipient with the second nucleic acid selected from the group of:
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:1, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:2, or its complement of the same length;
- a sequence which ran specifically hybridize to the sequence of SEQ ID NO:3, or its complement of the same length;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a gag protein, said sense or antisense sequence being selected from the group consisting of gag-encoding nucleotides of SEQ ID NO:1, gag-encoding nucleotides of SEQ ID NO:2, and gag-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2452-4939 or nucleotides 3112-4683 of SEQ ID NO:1, nucleotides 598-2169 of SEQ ID NO:2, or nucleotides 585-2156, of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a pol protein, said sense or antisense sequence being selected from the group consisting of pol-encoding nucleotides of SEQ ID NO:1, pot-encoding nucleotides of SEQ ID NO:2. and pol-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 4971-8060 of SEQ ID NO:1. nucleotides 2320-4737 of SEQ ID NO:2, or nucleotides 2307-5741 of SEQ ID NO:3;
- a nucleic acid sense or antisense sequence which encodes an env protein, said sense or antisense sequence being selected from the group consisting of env-encoding nucleotides of SEQ ID NO:1, env-encoding nucleotides of SEQ ID NO:2, and env-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2-1999 or nucleotides 86-1999 of SEQ ID NO:1, nucleotides 4738-6722 of SEQ ID NO:2, or nucleotides 5620-7533 of SEQ ID NO:3;
- wherein hybridization to the nucleic acid from the graft or organ correlates with the porcine retroviral infection in the graft or organ and hybridization to the nucleic acid from the recipient correlates with the porcine retroviral infection in the recipient, thereby localizing the origin of a porcine retroviral infection.
18 more elements
A method of screening a human subject for the presence or expression of an endogenous porcine retrovirus comprising:
- contacting a target nucleic acid derived from the human subject with a second nucleic acid selected from the group of:
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:1, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:2, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:3, or its complement of the same length;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a gag protein, said sense or antisense sequence being selected from the group consisting of gag-encoding nucleotides of SEQ ID NO:1, gag-encoding nucleotides of SEQ ID NO:2, and gag-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2452-4839 or nucleotides 3112-4683 of SEQ ID NO:1, nucleotides 598-2169 of SEQ ID NO:2, or nucleotides 585-2156, of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a pol protein, said sense or antisense sequence being selected from the group consisting of pol-encoding nucleotides of SEQ ID NO:1, pol-encoding nucleotides of SEQ ID NO,2, and pol-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 4971-8060 of SEQ ID NO:1, nucleotides 2320-4737 of SEQ ID NO:2, or nucleotides 2307-5741 of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes an env protein, said sense or antisense sequence being selected from the group consisting of env-encoding nucleotides of SEQ ID NO:1, env-encoding nucleotides of SEQ ID NO:2, and env-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2-1999 or nucleotides 86-1999 of SEQ ID NO:1, nucleotides 4738-6722 of SEQ ID NO:2, or nucleotides 5620-7533 of SEQ ID NO:3, under conditions in which the sequences can hybridize, hybridization being indicative of the presence of the endogenous porcine retrovirus or retroviral sequences in the human subject.
7 more elements
A method of determining the copy number or size of a porcine retrovirus, comprising:
- contacting a target nucleic acid from the donor, recipient, or a graft, with a second nucleic acid selected from the group of:
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:1, or its complement of the same length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:2, or its complement of the sane length;
- a sequence which can specifically hybridize to the sequence of SEQ ID NO:3, or its complement of the same length;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes a gag protein, said sense or antisense sequence being selected from the group consisting of gag-encoding nucleotides of SEQ ID NO:1, gag-encoding nucleotides of SEQ ID NO:2, and gag-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2452-4939 or nucleotides 3112-4683 of SEQ ID NO:1, nucleotides 599-2169 of SEQ ID NO:2, or nucleotides 585-2156, of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to sense or antisense sequence which encodes a pol protein, said sense or antisense sequence being selected from the group consisting of pol-encoding nucleotides of SEQ ID NO:1, pol-encoding nucleotides of SEQ ID NO:2, and pol-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 4871-8060 of SEQ ID NO:1, nucleotides 2320-4737 of SEQ ID NO:2, or nucleotides 2307-5741 of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence which encodes an env protein, said sense or antisense sequence being selected from the group consisting of env-encoding nucleotides of SEQ ID NO:1, env-encoding nucleotides of SEQ ID NO:2, and env-encoding nucleotides of SEQ ID NO:3;
- a nucleic acid which can specifically hybridize to a sense or antisense sequence from nucleotides 2-1999 or nucleotides 86-1999 of SEQ ID NO:1, nucleotides 4738-6722 of SEQ ID NO:2, or nucleotides 5620-7533 of SEQ ID NO:3.
7 more elements
Dependencies are inferred from the claim text.