Materials and methods relating to the attachment and display of substances on cell surfaces
AssigneeVlaams Interuniversitair Instituut voor Biotechnologie (VIB) vzw
InventorsLothar Steidler, Erik Remaut, Jeremy Mark Wells
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Abstract
Methods for obtaining surface expression of a desired protein or polypeptide in Gram-positive host organisms are provided. In addition, vectors useful in such methods as well as Gram-positive host organisms transformed with such vectors are disclosed.
Claims24
A recombinant vector including nucleic acid encoding a fusion protein comprising a secretion signal sequence obtained from the signal sequence of the protein usp45, a desired protein or polypeptide and a cell wall attachment domain comprising of at least the C-terminal anchoring domain obtained from staphylococcus aureus protein A so that when a lactococcus lactis host organism is transformed with the vector and the fusion protein is expressed, the desired protein or polypeptide is attached and displayed on the outer surface of the cell wall of the host.
A lactococcus lactis host organism transformed with a recombinant vector encoding a fusion protein comprising a secretion signal sequence, a desired protein or polypeptide and a cell wall attachment domain comprising at least the C-terminal anchoring domain obtained from staphylococcus aureus protein A so that when the fusion protein is expressed, the desired protein or polypeptide is attached and displayed on the outer surface of the cell wall of the host.
A method of engineering a lactococcus lactis host organism to display a desired protein or polypeptide on its surface comprising transforming lactococcus lactis with recombinant nucleic acid encoding a fusion protein comprising a secretion signal sequence, a desired protein or polypeptide and a cell wall attachment domain comprising at least the C-terminal anchoring domain obtained from Staphylococcus aureus protein A, so that lactococcus lactis is capable of expressing the fusion protein, whereby the cell wall attachment domain enables the lactococcus lactis to attach and display the desired protein or polypeptide on the cell wall surface.
Dependencies are inferred from the claim text.