Methods of using Flt-3 ligand for exogenous gene transfer
AssigneeImmunex Corporation
InventorsStewart D. Lyman, M. Patricia Beckmann
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Abstract
Ligands for flt3 receptors capable of transducing self-renewal signals to regulate the growth, proliferation or differentiation of progenitor cells and stem cells are disclosed. The invention is directed to flt3-L as an isolated protein, the DNA encoding the flt3-L, host cells transfected with cDNAs encoding flt3-L, compositions comprising flt3-L, methods of improving gene transfer to a mammal using flt3-L, and methods of improving transplantations using flt3-L. Flt3-L finds use in treating patients with anemia, AIDS and various cancers.
Claims24
A method of transfecting an exogenous gene into an hematopoietic cell comprising the steps of:
- (a) culturing the hematopoietic stem or progenitor cell in media comprising an effective amount of a flt3-L polypeptide;
- and
- (b) transfecting the cultured cells from step (a) with the gene.
A method of transferring an exogenous gene to a mammal comprising the steps of:
- (a) culturing hematopoietic stem or progenitor cells in media comprising an effective amount of a flt3-L polypeptide;
- (b) transfecting the cultured cells from step (a) with the gene;
- and
- (c) administering the transfected cells to the mammal.
Dependencies are inferred from the claim text.