Viral encoded semaphorin protein receptor polypeptides
AssigneeImmunex Corporation
InventorsMelanie K. Spriggs, Michael R. Comeau, Robert F. DuBose, Richard S. Johnson
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Abstract
The invention is directed to VESPR polypeptides as a purified and isolated protein, the DNA encoding the VESPR polypeptide, host cells transfected with cDNAs encoding VESPR, and methods for preparing VESPR polypeptides.
Claims27
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated polypeptide comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:2, wherein the percent identity is calculated using the GAP program with a unary comparison matrix, a 3.0 gap penalty, an additional 0.10 penalty for each symbol in each gap, and no penalty for end gaps, and the polypeptide binds a polypeptide selected from the group consisting of A39R semaphorin and AHV semaphorin.
An isolated polypeptide encoded by DNA selected from the group consisting of:
- (a) cDNA of SEQ ID NO:1;
- and
- (b) DNA sequences that hybridize under moderately stringent conditions of 55° C. in 5 X SSC, overnight, to the cDNA of (a);
- and which DNA sequences encode a polypeptide that binds a polypeptide selected from the group consisting of A39R semaphorin and AHV semaphorin.
A soluble polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) amino acids x1 to 945, inclusive, of SEQ ID NO:2, wherein x1 is amino acid 1 or 35;
- (b) a fragment of the sequence of (a), wherein the fragment binds a polypeptide selected from the group consisting of A39R semaphorin and AHV semaphorin.
A soluble polypeptide comprising an amino acid sequence that is at least 90% identical to an amino acid sequence selected from the group consisting of
- (a) amino acids x1 to 945 of SEQ ID NO:2, wherein x1 is amino acid 1 or 35 and,
- (b) a fragment of the sequence of (a);
- wherein the percent identity is calculated using the GAP program with a unary comparison matrix, a 3.0 gap penalty, an additional 0.10 penalty for each symbol in each gap, and no penalty for end gaps, and, wherein the soluble polypeptide binds a polypeptide selected from the group consisting of A39R semaphorin and AHV semaphorin.
A soluble polypeptide having the sequence of amino acids 380 to 482 of SEQ ID NO:2 and which binds a polypeptide selected from the group consisting of A39R semaphorin and AHV semaphorin.
An isolated polypeptide having the sequenc of amino acids 966 to 1568 of SEQ ID NO:2 and which binds a polypeptide selected from the group consisting of A39R semaphorin and AHV semaphorin.
Dependencies are inferred from the claim text.