Vectorial cloning system of DNA
AssigneeNovember AB Novus Medicatus Berling Gesellschaft fur Molekulare Medizin
InventorWolf Bertling
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Abstract
The invention relates to a vectorial cloning system consisting of a sequence of nucleotides, containing a fusion sequence and one or several restriction endonucleases, recognition sites for restriction endonucleases cutting outside their recognition sites, in addition to containing one or several other restriction endonuclease recognition sites which can be used to clone a foreign protein as well as the sequence for the desired foreign protein, wherein the foreign protein sequence is directly located on the fusion sequence after a subsequent restriction with the restriction endonucleases, followed by religation.
Claims10
A vector system for cloning comprising
- (a) a sequence encoding an affinity element,
- (b) one or more restriction endonuclease recognition sites for restriction endonucleases which cut outside their recognition sites,
- (c) one or more further restriction endonuclease recognition sites which can be used for cloning nucleic acid sequences encoding a desired foreign protein, and
- (d) a sequence encoding said foreign protein,
- wherein said restriction endonuclease recognition site(s) for restriction endonucleases which cut outside their recognition sites is/are designed such that, after restriction, the sequence encoding said foreign protein can be religated directly to said sequence encoding said affinity element.
Dependencies are inferred from the claim text.