Pectate lyases
AssigneeNovo Nordisk A/S
InventorsLene Nonboe Andersen, Martin Schülein, Niels Erik Krebs Lange, Mads Eskelund Bjørnvad, Søren Møller, Sanne O. Schrøder Glad, Markus Sakari Kauppinen, Kirk Schnorr, Lars Kongsbak
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Abstract
A novel group of pectate lyases comprising the amino acid sequence Asn Leu Asn Ser Arg Val Pro (NLNSRVP) belonging to Family 1 of polysaccharide lyases have good performance in industrial processes under neutral or alkaline conditions such as laundering and textile processing. The pectate lyase are derivable from Bacillus species.
Claims23
An isolated pectate lyase comprising a first amino acid sequence corresponding to residues 236-242 of SEQ ID NO:2, wherein said pectate lyase is derived from a Bacillus species.
An isolated pectate lyase selected from the group consisting of:
- i) a polypeptide comprising an amino acid sequence as shown in positions 27-359 of SEQ ID NO:2, and
- (ii) a polypeptide having pectate lyase activity encoded by a nucleic acid that hybridizes with the nucleotide sequence of SEQ ID NO:1 under hybridization conditions comprising 5×SSC at 45° C. and washing conditions comprising 2×SSC at 60° C.
An isolated pectate lyase which is selected from the group consisting of:
- i) a polypeptide comprising an amino acid sequence as shown in positions 28-341 of SEQ ID NO:4, and
- (ii) a polypeptide having pectate lyase activity encoded by a nucleic acid that hybridizes with the nucleotide sequence of SEQ ID NO:3 under hybridization conditions comprising 5×SSC at 45° C. and washing conditions comprising 2×SSC at 60° C.
An isolated pectate lyase which is selected from the group consisting of:
- i) a polypeptide comprising an amino acid sequence as shown in positions 181-509 of SEQ ID NO:6, and
- (ii) a polypeptide having pectate lyase activity encoded by a nucleic acid that hybridizes with the nucleotide sequence of SEQ ID NO:5 under hybridization conditions comprising 5×SSC at 45° C. and washing conditions comprising 2×SSC at 60° C.
An isolated pectate lyase which is selected from the group consisting of:
- i) a polypeptide comprising an amino acid sequence as shown in positions 42-348 of SEQ ID NO:8, and
- (ii) a polypeptide having pectate lyase activity encoded by a nucleic acid that hybridizes with the nucleotide sequence of SEQ ID NO:7 under hybridization conditions comprising 5×SSC at 45° C. and washing conditions comprising 2×SSC at 60° C.
An isolated pectate lyase which is selected from the group consisting of:
- i) a polypeptide comprising an amino acid sequence as shown in positions 25-335 of SEQ ID NO:10, and
- (ii) a polypeptide having pectate lyase activity encoded by a nucleic acid that hybridizes with the nucleotide sequence of SEQ ID NO:9 under hybridization conditions comprising 5×SSC at 45° C. and washing conditions comprising 2×SSC at 60° C.
An isolated polypeptide selected from the group consisting of:
- a) a polypeptide having pectate lyase activity and comprising an amino acid sequence as shown in SEQ ID NO:2 from residue 27 to residue 359;
- b) a polypeptide having pectate lyase activity and comprising an amino acid sequence as shown in SEQ ID NO:4 from residue 28 to residue 241;
- c) a polypeptide having pectate lyase activity and comprising an amino acid sequence as shown in SEQ ID NO:6 from residue 181 to residue 509;
- d) a polypeptide having pectate lyase activity and comprising an amino acid sequence as shown in SEQ ID NO:8 from residue 42 to residue 348;
- and
- e) a polypeptide having pectate lyase activity and comprising an amino acid sequence as shown in SEQ ID NO:10 from residue 25 to residue 335.
A fused polypeptide comprising (i) a polypeptide having pectate lyase activity which is linked to (ii) one or more cellulose binding domains (CBD), wherein said polypeptide having pectate lyase activity comprises the amino acid sequence of SEQ ID NO:12.
Dependencies are inferred from the claim text.