Methods and reagents for in situ amplification
AssigneeAkzo Nobel N.V.
InventorsYuexia Li, Kathy Lynn Hickman, Eun Mi Lee, Gregory John Hurteau
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Abstract
The present invention provides in situ amplification methods for the detection of a target nucleic acid molecule. The methods use a molecule termed “molecular tape” to retain the amplified target nucleic acid molecule within a cell upon amplification. The molecular tape, which may be used with a variety of amplification methods, improves the in situ detection of an amplified target molecule.
Claims29
A method for preventing an amplified target nucleic acid molecule from leaking from a cell, comprising the step of exposing the cell to molecular tape, which is a nucleic acid or a nucleic acid analog and does not function as a primer, wherein the molecular tape enters the cell and hybridizes with the amplified molecule to form a complex, and leakage is thereby prevented.
Dependencies are inferred from the claim text.