Method for identifying inhibitors of JAK2/cytokine receptor binding
AssigneePharmacia & Upjohn Company
InventorsSteven L. Roberds, Paul S. Kaytes
Life of this patent
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Abstract
The invention provides a method of identifying a therapeutic useful for treating or preventing asthma, which method includes the steps of contacting a identify compounds that inhibit the binding of human JAK2 protein to the βc first molecule comprising at least the N terminal 294 residues of JAK2 protein as shown in SEQ ID NO: 5 with a second molecule comprising at least 13 membrane proximal cytoplasmic amino acid residues of the βc subunit of the IL-3, IL-5, and GM-CSF receptors as shown in SEQ ID NO: 3 in the presence of a candidate compound to subunit of the IL-3, IL-5 and GM-CSF receptors. Compounds that block the signalling pathways of the IL-3, IL-5 and GM-CSF receptors may be of use in the treatment of asthma.
Claims22
A method of screening for compounds useful for inhibiting JAK2/Cytokine receptor binding, said method comprising:
- (a) contacting a first molecule comprising at least the N-terminal 294 residues of JAK2 protein as shown in (SEQ ID NO:5) with a second molecule comprising at least 13 membrane-proximal cytoplasmic amino acids of βc subunit of the IL-3, IL-5 and GM-CSF receptors as shown in (SEQ ID NO:2) in the presence of a candidate compound;
- and
- (b) detecting whether a complex forms between said first and second molecules to determine whether said candidate compound inhibits the formation of said complex.
A method for screening compounds useful for inhibiting JAK2/Cytokine receptor binding, said method comprising:
- (a) contacting a first molecule comprising at least the N terminal 294 residues of JAK2 protein as shown in (SEQ ID NO:
- 5) with a second molecule comprising a polypeptide selected from the group consisting of polypeptides represented by SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 15, SEQ ID NO:
- 17, SEQ ID NO:
- 18 and SEQ ID NO:
- 19 in the presence of a candidate compound;
- and
- (b) detecting whether a complex forms between said first and second molecules to determine whether said candidate compound inhibits the formation of said complex.
Dependencies are inferred from the claim text.