Method of isolating cells
OwnerIan Kenneth O'Neill, Alexandre Loktionovinventors
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Abstract
The invention provides a method of isolating cells from a faecal stool, the method comprising the steps of a) cooling the stool to a temperature below its gel freezing point, and b) removing cells from the stool whilst maintaining the stool at a temperature below its gel freezing point such that the stool remains substantially intact. The invention further provides methods of purifying cells comprising use of immunomagnetic beads and/or boric acid. Cells isolated according to the invention may be used in diagnostic tests and assay procedures for monitoring a biological or biochemical property of tissue.
Claims21
A method of isolating cells from a faecal stool, the method comprising the steps of:
- a) cooling the stool to a temperature below its gel freezing point;
- b) removing cells from the stool while maintaining the stool at a temperature below its gel freezing point, such that the stool remains substantially intact.
A method of isolating cells from a fecal stool comprising the steps of:
- a) cooling the stool to a temperature below its gel freezing point;
- and
- b) washing cells from the surface of the stool with an aqueous solution, while maintaining the stool at a temperature below its gel freezing point, such that the stool remains substantially in tact.
A method of isolating cells from a faecal stool, the method comprising the steps of:
- a) cooling the stool to a temperature below its gel freezing point in the range −80° C. to 15° C.;
- and
- b) washing cells from the surface layers of the stool with an aqueous solution while maintaining the stool at a temperature below its gel freezing point such that the stool remains substantially intact, wherein the aqueous solution contains a fatty acid having from one to six carbon atoms or a salt thereof, thereby providing an aqueous suspension of cells.
Dependencies are inferred from the claim text.