Methods for rapid cloning for full length cDNAs using a pooling strategy

A method for high throughput full length cDNA cloning comprising:

• (a) preparing cDNAs from mRNAs;
• (b) size selecting the cDNAs into discrete n ranges;
• (c) transforming host cells with cDNAs from the each discrete n size ranges to make n separate sub-libraries;
• (d) placing up to m number of clones from each n sub-libraries into individual wells of k numbers of microtiter plates, each microtiter plates containing x numbers of rows and y numbers of columns, in a fashion that clones from each n sub-library will go into n separate sets of microtiter plates within the total k number of microtiter plates;
• (e) pooling all the clones from individual microtiter plates, and placing each pool of clones into wells of a second separate microtiter plate containing i rows and j columns, the second microtiter plate containing a total of k number of wells in such a fashion that placement of pools of clones will be placed in separate n regions within the microtiter plate in order to facilitate the identification of cDNA insert sizes;
• (f) identifying the well location of cDNA of interest in the second microtiter plate which has the longest insert;
• and correlating the well located to one of the earlier k number of microtiter plates;
• and
• (g) identifying the well location in the microtiter correlated in step (f) for the cDNA of interest;
• and in which m is a positive integer equal or greater than one;
• n, k, x, y, i, and j are positive integers greater than one;
• with the proviso i times j equals k.