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US 6,187,314 B1Granted
Ginkgo biloba composition method to prepare the same and uses thereof
AssigneeShanghai Inst. of Chinese Materia Medica
InventorsDe Long Xie, Ning Wang, Oi Gao, Guo An Zhang, Bao Ping Shao, Xiao Wu Jin, Xin Sheng Huang
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Abstract
This invention provides different compositions extracted from Ginkgo biloba leaves. Said compositions comprise new active components. This invention also provides a method of preparation of the compositions and individual components of said compositions. Finally, this invention provides various uses of this composition.
Claims6
A method for obtaining a Ginkgo biloba composition containing flavonoids and lactone, and determining the total amount of flavonoids therein comprising steps of:
- (a) obtaining dried Ginkgo biloba leaves;
- (b) breaking the leaves into small pieces;
- (c) putting the broken leaves through reflux in a solution selected from the group consisting of water, C1 to C3 alkanols, acetone, and a combination thereof under conditions permitting the extraction of flavonoids and lactones to produce an extract and a residue;
- (d) separating the extract from the residue;
- (e) concentrating the separated extract to a density of about 1.2 to about 1.25 at about 60° C.;
- (f) applying the concentrate from step (e) to at least two kinds of resins under conditions permitting binding of flavonoids and lactones;
- (g) eluting the bound flavonoids and lactones, thereby producing an extract composition containing flavonoids and lactones from Ginkgo biloba;
- and
- (h) determining the total amount of the flavonoids in the eluted composition of step (g) comprising a standard curve assay calculation wherein rutin is used as the standard.
6 more elements
Dependencies are inferred from the claim text.