Engineered acarid allergen and process for producing the same
AssigneesAsahi Breweries, Ltd., Torii Pharmaceuticals Co., Ltd., The Nikka Whisky Distilling Co., Ltd.
InventorsChiharu Nishiyama, Toshifumi Yuuki, Yasushi Okumura
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Abstract
The invention provides a method for producing a modified major mite allergen, obtained by altering a major allergen of house dust mites by gene engineering, which involve the steps of culturing prokaryotic or eukaryotic host cells transformed with a replication vector containing a gene encoding modified major mite allergen expressed from a promoter and collecting the expressed modified major mite allergen from the culture. The present invention provides a modified major mite allergen produced by the method, DNA molecules encoding same, and a pharmaceutical composition containing the modified major mite allergen.
Claims6
An isolated DNA molecule comprising a nucleotide sequence encoding a modified major mite allergen, wherein said nucleotide sequence is modified from nucleotide sequences SEQ ID NO:1, SEQ ID NO:3, or SEQ ID NO:5, encoding wild-type major mite allergen Derf II, to substitute a codon for a different amino acid residue other than alanine at a residue position selected from the group consisting of the 7th, 9th, 19th, 128th, and 129th amino acid residue of wild-type major mite allergen Derf II.
Dependencies are inferred from the claim text.