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US 6,184,449 B2Granted
1-Aminocyclopropane-1-carboxylate synthase genes from rosa to control ethylene levels in roses
AssigneeTagawa Greenhouses, Inc.
InventorRajinder S Ranu
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Abstract
A gene which encodes an ACC synthase is isolated from rose plants, specifically Rosa (cardinal red). This gene is modified for expression in transgenic plants. Isolation of high quality mRNA for gene isolation is achieved through use and adaptation of a 2-butoxyethanol precipitation technique using large amount of initial tissue in order to achieve critical mass for precipitation.
Claims13
An isolated DNA molecule consisting of SEQ ID NO:
- 1.
A vector useful when introduced into a rose plant cell, comprising a polynucleotide hang the nucleotide sequence of SEQ ID NO:
- 1, wherein the polynucleotide is inserted into the vector in reverse orientation.
Dependencies are inferred from the claim text.