Lysine rich protein from winged bean
AssigneesBeijing Vegetable Research Center, Institute of Botany, Academia Sinica, University of Hawaii
InventorsSamuel S. M. Sun, Liwen Xiong, Yuxiang Jing, Bolin Liu
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Abstract
A lysine-rich protein gene has been cloned from winged bean, Psophocarpus tetragonolobus, and its DNA sequence and deduced protein sequence are provided. Methods for transforming plant cells are provided that mature into plants having elevated levels of lysine in their seeds.
Claims18
A recombinant nucleic acid in isolated and purified form which nucleic acid comprises a nucleotide sequence encoding a winged bean lysine-rich protein (WBLRP), wherein said protein (a) has the amino acid sequence set forth in SEQ ID NO:2 or (b) is encoded by a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence complementary to that which encodes the protein of SEQ ID NO:2 and wherein said protein is naturally expressed only in the developing seeds and its expression is not related to pathogen activity or wounding.
An isolated nucleic acid that is fully complementary to the nucleotide sequence set forth in SEQ ID NO:1.
A recombinant DNA molecule comprising an expression system, which expression system comprises a nucleotide sequence encoding WBLRP, wherein said encoding sequence is under the transcriptional and/or translational control of regulatory sequences heterologous to said WBLRP-encoding nucleotide sequence, and wherein said protein (a) has the amino acid sequence set forth in SEQ ID NO:2 or (b) is encoded by a nucleotide sequence that hybridizes under stringent conditions to a nucleotide sequence complementary to that which encodes the protein of SEQ ID NO:2 and said protein is naturally expressed only in the developing seeds and its expression is not related to pathogen activity or wounding.
Dependencies are inferred from the claim text.