Purification of NGF
AssigneeGenentech, Inc.
InventorsLouis E. Burton, Charles H. Schmelzer, Joanne T. Beck
Life of this patent
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Abstract
Methods are provided for large scale purification of neurotrophins, including mature NGF, suitable for clinical use. The methods provide means to separate neurotrophins from various less desirable misprocessed, misfolded, size, glycosylated, or charge forms. Compositions of neurotrophins, including mature NGF, substantially free of these variants are also provided.
Claims23
A process to isolate a neurotrophin from a mixture comprising variants of said neurotrophin that can include a midfolded variant, an incorrectly proteolytically processed variant, and a glycosylation valiant of said neurotrophin, wherein the process comprises:
- a) loading the mixture onto a hydrophobic interaction chromatography resin;
- b) eluting the neutotrophin from the resin with an elution buffer inder conditions in which the neurotrophin separates from the variant;
- and
- c) collecting the neurotrophin.
A process to isolate a neurotrophin from a mixture of proteins, compnsing separating the neurotrophin from the mixture using a silica gel resin by elution of the neurotrophin with an eluition buffer consisting essentially of TMAC or TEAC buffered at about pH 3.5 to 8.0.
Dependencies are inferred from the claim text.