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AssigneesMillennium Pharmaceuticals, Inc., Theodor-Kocher Institute
InventorsMarcel Loetscher, Bernhard Moser, Shixin Qin, Charles R. Mackay
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The present invention relates to proteins or polypeptides, referred to herein as isolated and/or recombinant mammalian (e.g., human) IP-10/Mig receptor proteins designated CXC Chemokine Receptor 3 (CXCR3) and variants thereof, including those characterized by selective binding of one or more chemokines (e.g., IP-10 and/or Mig), and/or the ability to induce a cellular response (e.g., chemotaxis, exocytosis). Antibodies reactive with CXCR3 receptors can be produced using the proteins or variants thereof or host cells comprising same as immunogen. Another aspect of the invention relates to isolated and/or recombinant nucleic acids encoding a mammalian (e.g., human) CXCR3 protein and variants thereof, including antisense nucleic acid, recombinant nucleic acid constructs, such as plasmids or retroviral vectors, comprising a nucleic acid which encodes a protein of the present invention or variant thereof, and to host cells comprising a nucleic acid or construct, useful in the production of recombinant proteins. Also encompassed are methods of identifying ligands, and inhibitors (e.g., antagonists) or promoters (e.g., agonists) of receptor function, including methods in which host cells comprising a nucleic acid encoding a CXCR3 or variant thereof are used in an assay to identify and assess the efficacy of ligands, inhibitors or promoters. Inhibitors and promoters of receptor function can be used to modulate receptor activity, permitting selective inhibition of lymphocyte function, particularly of effector cells such as activated T lymphocytes and NK cells for therapeutic purposes.
An antibody or antigen-binding fragment thereof which binds a human CXC Chemokine Receptor 3 (CXCR3) protein having the amino acid sequence of SEQ ID NO:2.
An antibody or antigen-binding fragment thereof which binds a human CXC Chemokine Receptor 3 (CXCR3) protein having the amino acid sequence of SEQ ID NO:2, wherein said antibody or fragment can inhibit one or more functions of said human CXCR3 protein selected from the group consisting of binding of a ligand, signalling activity and cellular response function.
An antibody or antigen-binding fragment thereof which binds a human CXC Chemokine Receptor 3 (CXCR3) protein having the amino acid sequence of SEQ ID NO:2, wherein said antibody or fragment selectively inhibits the interaction of a human CXCR3 protein with IP-10.
Monoclonal antibody 1C6 (ATCC Acession Number HB-12330) or an antigen-binding fragment thereof which binds a human CXC Chemokine Receptor 3 (CXCR3) protein having the amino acid sequence of SEQ ID NO:2.
An antibody or antigen-binding fragment thereof, wherein said antibody or fragment can inhibit binding of human IP-10 to a human CXC Chemokine Receptor 3 (CXCR3) protein and can inhibit one or more functions mediated by said CXCR3 protein in response to IP-10 binding selected from the group consisting of signalling activity and cellular response function, wherein said antibody or fragment can bind a human CXCR3 protein having the amino acid sequence of SEQ ID NO:2.
A method of detecting a human CXC Chemokine Receptor 3 (CXCR3) protein in a sample comprising:
Hybridoma cell line HB-12330.
The antibody produced by hybridoma cell line HB-12330 or an antigen-binding fragment thereof.
An antibody or antigen-binding fragment thereof which binds a human CXC Chemokine Receptor 3 (CXCR3) protein having the amino acid sequence of SEQ ID NO:2, wherein said antibody or fragment can inhibit one or more functions of said human CXCR3 protein selected from the group consisting of binding of a ligand, signalling activity and cellular response function, and wherein the binding of said antibody or antigen-binding fragment to said human CXCR3 can be inhibited by a polypeptide having a sequence which is the same as that of residues 1-15 of SEQ ID NO:2.
An antibody or antigen-binding fragment thereof which binds a human CXC Chemokine Receptor 3 (CXCR3) protein having the amino acid sequence of SEQ ID NO:2, wherein said antibody or fragment can inhibit one or more functions of said human CXCR3 protein selected from the group consisting of binding of a ligand, signalling activity and cellular response function, and wherein the binding of said antibody or antigen-binding fragment to said human CXCR3 can be inhibited by a polypeptide having a sequence which is the same as that of residues 16-30 of SEQ ID NO:2.
A method of detecting a human CXC Chemokine Receptor 3 (CXCR3) protein in a sample comprising:
Dependencies are inferred from the claim text.