Hippocampus-associated proteins, DNA sequences coding therefor and uses thereof
AssigneeBTG International Limited
InventorsRichard Lathe, Kenneth Andrew Rose, Genevieve Stapleton
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Abstract
This invention provides novel hippocampus-associated proteins and DNA sequences coding therefor. In an investigation of hippocampus-associated proteins by differential screening of a rat hippocampus cDNA library, a cDNA species encoding a novel protein designated Hct-1 was isolated and shown to be a to cytochromes P450. The use of hybridization probes based on the rat Hct-1 sequence has led to the identification of homologues in other mammalian species.
Claims8
A isolated cytochrome P450 protein, at least a portion of which is encoded by a DNA molecule selected from the following:
- (a) DNA molecules containing the coding sequence set forth in SEQ ID NO:1 beginning at nucleotide 1 and ending at nucleotide 1242,
- (b) DNA molecules containing the coding sequence set forth in SEQ ID NO:3 beginning at nucleotide 81 and ending at nucleotide 1601,
- (c) DNA molecules comprising a Cyp7b protein coding sequence and which are capable of hybridizing with the DNA molecule defined in (a) or (b) under standard hybridization conditions defined as 2×SSC at 65° C.
- (d) Cytochrome P450-encoding DNA molecules capable of hybridizing with the DNA molecule defined in (a), (b) or (c) under reduced stringency conditions defined as 6×SSC at 65° C.
An isolated protein selected from the following:
- (i) the protein designated rat Cyp7b comprising the amino acid sequence set forth in SEQ ID NO:2,
- (ii) the protein designated mouse Cyp7b comprising the amino acid sequence set forth in SEQ ID NO:4, and
- (ii) the protein designated as human Cyp7b comprising the amino acid sequence set forth in SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.