US 6,184,347 B2 · Minimization of blooming in high-density arrays by using reactive wash reagents

Abstract

A wash reagent employed for the bulk washing of the surface of a high-density array to remove unreacted reactants from cells of the array while, at the same time, reacting with the unreacted monomer in order to prevent reaction of the reacted monomer with functional groups on the surface of the HDA outside of the region of the surface to which the reactive monomer is applied. The wash reagent is chosen for a particular solid-state synthesis so that the unreacted reactants and catalyzing agents are soluble in the wash reagent, so that the wash reagent does not react with, or catalyze, reactions of the substrate or the biopolymers bound to the substrate, and so that the wash reagent reacts with unreacted reactive monomer in order to prevent subsequent reactions of the unreacted reactive monomer.

Claims15

Dependent claims

A method used, during the solid-state synthesis of surface-bound polymers on a solid substrate, for removing a reaction solution including unreacted reactive reagents from the surface of the solid substrate and from any nascent polymers bound to the solid substrate, the method comprising:

selecting a reactive wash solution that is not reactive toward, and does not catalyze reactions with, the solid substrate or any nascent polymers bound to the solid substrate but that reacts with, and deactivates, the reactive reagents and that is miscible with the reaction solution;
and
applying the reactive wash solution to the surface of the solid substrate in order to react with, and deactivate, any unreacted reactive reagents and to remove the reaction solution from the surface of the solid substrate and from any nascent polymers bound to the substrate.

A method for coupling a reactive monomer molecule to a nascent polymer bound to surface of a solid substrate, the method comprising:

applying monomer molecules and any reagents required to catalyze the coupling of the reactive monomer molecule with the nascent polymer to the surface of the solid substrate;
and
applying a reactive wash solution to the surface of the solid substrate to react with, and deactivate, any remaining reactive monomers on the surface of the solid substrate and to dissolve and remove the remaining deactivated monomers and any reagents required to catalyze the coupling of the reactive monomer molecule to the polymer from the surface of the solid substrate and from any synthesized polymers bound to the substrate.

Dependencies are inferred from the claim text.

Minimization of blooming in high-density arrays by using reactive wash reagents

Life of this patent

Minimization of blooming in high-density arrays by using reactive wash reagents

Life of this patent

Abstract

Claims15