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US 6,184,254 B2Granted
Methods of identifying modulators of perivascular sensory nerve Ca2+ receptors
AssigneeBoard of Regents, The University of Texas System
InventorsRichard D. Bukoski, Ka Bian
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Abstract
Methods of identifying compounds that relax or stimulate arterial tension through their action on perivascular sensory nerve calcium receptors are described. Compounds identified through such methods are useful for the treatment of hypertension, hypotension and other diseases and conditions that alter normal physiological blood pressure.
Claims6
A method of screening for a compound that reduces vascular tone via a Ca2+ receptor of a perivascular sensory nerve comprising:
- (a) contracting a mesenteric artery freed of endothelial tissue and measuring its arterial tension;
- (b) contacting the mesenteric resistance artery with a first compound;
- (c) measuring arterial relaxation of the mesenteric resistance artery after contact with the first compound;
- (d) repeating step (b) but after pre-treatment of the mesenteric resistance artery with a second compound that blocks Ca2+ receptor-mediated activity;
- and
- (e) comparing the levels of arterial tension in step (a) with the levels in step (c), wherein the first compound reduces vascular tone via Ca2+ receptor of a perivascular sensory nerve if the level of step (c) is lower than the level of step (a) and if step (d) prevents reduction in arterial tension by the first compound.
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Dependencies are inferred from the claim text.