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US 6,184,200 B2Granted
Truncated glial cell line-derived neurotrophic factor
AssigneeAmgen Inc.
InventorShaw-Fen Sylvia Hu
Life of this patent
Estimating the term…
Abstract
Disclosed are novel proteins, referred to as truncated glial cell line-derived neurotrophic factor (truncated GDNF) proteins, that promote dopamine uptake by dopaminergic cells and promote the survival of nerve cells. Also disclosed are processes for obtaining the truncated GDNF proteins by recombinant genetic engineering techniques.
Claims21
A truncated glial cell line-derived neurotrophic factor (GDNF) protein product consisting of an amino acid sequence
- X—(Cys41-Cys133)—Y
- wherein
- (Cys41-Cys133) consists of Cys41 through Cys133 of SEQ ID NO:2;
- Y represents the carboxy terminal group of Cys133, a carboxy-terminus amino acid residue of Ile134, or a substituted amino acid residue, and
- X represents a methionylated or nonmethionylated amine group of Cys41 or amino-terminus amino acid residue(s) selected from the group:
- G RG NRG (SEQ ID NO:3) KNRG (SEQ ID NO:4) GKNRG (SEQ ID NO:5) RGKNRG (SEQ ID NO:6) QRGKNRG (SEQ ID NO:7) GQRGKNRG (SEQ ID NO:8) RGQRGKNRG (SEQ ID NO:9) RRGQRGKNRG (SEQ ID NO:10) G RRGQRGKNRG (SEQ ID NO:11) KG RRGQRGKNRG (SEQ ID NO:12) GKG RRGQRGKNRG (SEQ ID NO:13) RGKG RRGQRGKNRG (SEQ ID NO:14) SRGKG RRGQRGKNRG (SEQ ID NO:15) NSRGKG RRGQRGKNRG (SEQ ID NO:16) ENSRGKG RRGQRGKNRG (SEQ ID NO:17) PENSRGKG RRGQRGKNRG (SEQ ID NO:18) NPENSRGKG RRGQRGKNRG (SEQ ID NO:19) ANPENSRGKG RRGQRGKNRG (SEQ ID NO:20) A ANPENSRGKG RRGQRGKNRG (SEQ ID NO:21) AA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:22) AAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:23) QAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:24) RQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:25) NRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:26) RNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:27) ERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:28) RERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:29) RRERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:30) P RRERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:31) LP RRERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:32) VLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:33) AVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:34) MAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:35) QMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:36) KQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG (SEQ ID NO:37) DKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG and (SEQ ID NO:38) PDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG
- or a substitution or deletion variant of X, wherein said variant is in excess of 70% identical to an amino acid sequence of X as set forth above when four gaps in a length of 100 amino acids may be introduced to assist in that alignment, and
- wherein said glial cell line-derived neurotrophic factor protein product has a neurotrophic effect on dopaminergic nerve cells.
5 more elements
Dependencies are inferred from the claim text.