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OwnerØystein Fodstad, Gunnar Kvalheiminventors
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The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.
A method for detecting and quantitating a specific living target cell in a cell suspension of a mixed cell population at a sensitivity of one target cell per 100 or more total cells, in a fluid system containing a mixed cell population, or in a single-cell suspension prepared from a solid tissue, with the exception of normal and malignant hematopoietic cells in blood and bone marrow, the method comprising the steps of:
A method for detecting a specific living target cell in a cell suspension of a mixed cell population at a sensitivity of one target cell per 100 or more total cells, in a fluid system containing a mixed cell population, or in a single cell suspension prepared from a solid tissue, with the exception of normal and malignant hematopoietic cells in blood and bone marrow, the method comprising the steps of:
Dependencies are inferred from the claim text.