Method for reducing hook effect in an immunoassay
AssigneeBoehringer Mannheim GmbH
InventorsUlrich Neumann, Helmut Lenz, Norbert Franken
Life of this patent
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Abstract
In order to extend the measuring range and to reduce the Hook effect in an immunological method of determination of an antigen based on the principle of a sandwich assay it is preferable to use a method which is characterized in that the labelled detection molecule is an oligomer of a binding molecule selected from antibody or/and antibody fragment.
Claims19
A method for extending the concentration range at which an analyte can be detected in a sample liquid according to the principle of a sandwich assay comprising the step of
- a) incubating the sample liquid in the presence of a solid phase with at least two receptors that are each capable of binding to the analyte wherein the first receptor is soluble and the second receptor (i) is bound to a solid phase or (ii) is capable of binding to a solid phase;
- and
- b) detecting the analyte by determining a label in the solid phase, the liquid phase, or both the solid phase and the liquid phase,
- wherein the first receptor is a labeled oligomer of a binding molecule selected from antibodies, antibody fragments, and mixtures thereof, and wherein all of said antibodies and antibody fragments bind to the same antigen, in an amount sufficient to reduce the Hook Effect.
Method for reducing Hook effect in an immunoassay, comprising carrying out said immunoassay with an oligomeric antibody, wherein said oligomeric antibody comprises a covalently linked conjugate of at least two antibodies, antibody fragments, and/or mixtures therof, the degree of oligomerization being 2-15, wherein all of said antibodies and antibody fragments bind to the same antigen, in an amount sufficient to reduce the Hook effect.
Dependencies are inferred from the claim text.