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US 6,184,037 B2Granted
Chitosan related compositions and methods for delivery of nucleic acids and oligonucleotides into a cell
AssigneeGenemedicine Co., Ltd.
InventorsAlain Rolland, Russell J. Mumper
Life of this patent
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Abstract
Compositions of chitosan-based compounds and nucleic acid or oligonucleotide which are capable of delivery to a cell. Methods of preparation of the compositions. Methods of administering the compositions in vitro to cells in culture or in vivo to an organism.
Claims23
A method of making a composition comprising:
- (a) a chitosan-based compound;
- and (b) a nucleic acid or an oligonucleotide, wherein said composition delivers said nucleic acid or said oligonucleotide into a cell, wherein said composition has a net negative charge ratio, comprising the steps of:
- (a) exposing said chitosan-based compound to an acid;
- (b) filtering the acid treated product of step (a);
- (c) adding the acid treated and filtered product of step (b) to said nucleic acid or said oligonucleotide in an acceptable pharmaceutical carrier.
2 more elements
A composition comprising:
- (a) a chitosan-based compound;
- (b) a cryoprotectant;
- and
- (c) a nucleic acid or an oligonucleotide, wherein said composition delivers said nucleic acid or said oligonucleotide into a cell, and wherein said composition has a net negative charge ratio.
1 more element
Dependencies are inferred from the claim text.