Ornithine biosynthesis enzymes
AssigneeE. I. Du Pont de Nemours and Company
InventorsRebecca E. Cahoon, J. Antoni Rafalski, Saverio Carl Falco
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Abstract
This invention relates to an isolated nucleic acid fragment encoding an ornithine-oxo-acid transaminase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the ornithine-oxo-acid transaminase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the ornithine-oxo-acid transaminase in a transformed host cell.
Claims7
An isolated nucleic acid fragment comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding an orthinine-oxo-acid transaminase that is at least 500 contiguous nucleotides that hybridizes under highly stringent conditions to an isolated nucleic acid fragment encoding the amino acid sequence set forth in SEQ ID NO:2, wherein said highly stringent conditions is defined by washes with 6× SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2× SSC, 0.5% SDS at 45° C. for 30 min, and two washes in 0.1× SSC, 0.1% SDS at 65° C.;
- (b) an isolated nucleic acid fragment encoding an orthinine-oxo-acid transaminase that is at least 250 nucleotides that hybridizes under highly stringent conditions to an isolated nucleic acid fragment encoding the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, and SEQ ID NO:10, wherein said highly stringent conditions is defined in (a);
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- (c) an isolated nucleic acid fragment that is fully complementary to the nucleic acid fragment of (a) or (b).
An isolated nucleic acid fragment comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding an orthinine-oxo-acid transaminase that is at least 500 contiguous nucleotides that hybridizes under highly stringent conditions to an isolated nucleic acid fragment having a nucleotide sequence set forth in SEQ ID NO:1, wherein said highly stringent conditions is defined by washes with 6× SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2× SSC, 0.5% SDS at 45° C. for 30 min, and two washes in 0.1× SSC, 0.1% SDS at 65° C.;
- (b) an isolated nucleic acid fragment encoding an orthinine-oxo-acid transaminase that is at least 250 nucleotides that hybridizes under highly stringent conditions to an isolated nucleic acid fragment having a nucleic acid sequence set forth in a member selected from the group consisting of SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, and SEQ ID NO:9, wherein said highly stringent conditions is defined in (a);
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- (c) an isolated nucleic acid fragment that is fully complementary to the nucleic acid fragment of (a) or (b).
Dependencies are inferred from the claim text.