Catalytically-active gelatinase mutant
AssigneeWashington University
InventorRobert M. Senior
Life of this patent
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Abstract
A truncated mutant of the 92 kDa gelatinase is disclosed which is catalytically active comparable to the full-length enzyme but, unlike the full-length enzyme, is essentially inactive against insoluble elastin. The truncated mutant preferably comprises residues 107-217 fused to residues 391-443 of the parent molecule. The truncated mutant is useful for treatment of disorders requiring the removal of excess connective tissue.
Claims3
A truncated mutant, SEQ ID NO:1, of the 92 kDa gelatinase having a sequence as shown in FIG. 2 consisting of residues 106-216 fused to residues 391-443 of the parent molecule.
The method of modifying the 92 kDa gelatinase protein to make it essentially inactive against insoluble elastin but to remain catalytically active which comprises fusing amino acid residues 106-216 to residues 391-443 of the parent 92 kDa gelatinase protein molecule to thereby provide the truncated mutant having an amino acid sequence as shown in FIG. 2 (SEQ ID NO:1).
Dependencies are inferred from the claim text.