Polypeptides having prenylcysteine carboxyl methyltransferase activity or inhibiting activity
AssigneeNew York University
InventorMark R. Philips
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Abstract
A mammalian protein, prenylcysteine carboxyl methyltransferase, involved in further post-translational modification of prenylated proteins, is cloned, characterized, and expressed in non-native cells. Such membrane preparations can be used to screen for inhibitors of prenylcysteine carboxyl methyltransferase activity.
Claims21
An isolated polypeptide, comprising:
- a) the amino acid sequence of SEQ ID NO:5;
- b) an amino acid sequence having at least 70% identity to a) and having prenylcysteine carboxyl methyltransferase (pcCT) activity either when isolated or when membrane bound;
- c) a fragment of a) or b) which has pcCMT activity either when isolated or when membrane bound;
- or
- d) a protein encoded by a nucleotide sequence which hybridizes to the complement of nucleotides 44 to 895 of SEQ ID NO:4 in 50% formamide and 5×SSC at 40° C., followed by nonstringent washing, said protein having pcCMT activity either when isolated or when membrane bound.
An isolated polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:5, which fragment is capable of inhibiting the carboxyl methylation activity of the prenylcysteine carboxyl methyltransferase (pcCMT) of SEQ ID NO:5.
A membrane on which prenylcysteine carboxyl methyltransferase (pcCMT) is present, said pcCMT being non-reactive to said membrane and having the amino acid sequence of a protein encoded by a nucleotide sequence which hybridizes to the complement of nucleotides 44 to 895 of SEQ ID NO:4 in 50% formamide and 5×SSC at 40° C., followed by nonstringent washing, wherein said pcCMT on said membrane has pcCMT activity.
A membrane on which prenylcysteine carboxyl methyltransferase (pcCMT) is present, said pcCMT being non-native to said membrane and having an amino acid sequence having at least 70% identity to SEQ ID NO:5 and having pcCMT activity, or a fragment thereof having pcCMT activity.
Dependencies are inferred from the claim text.