Method of releasing solid matrix affinity adsorbed particulates
AssigneesCBD Technologies Ltd., Yissum R&D Company of the Hebrew University
InventorsDaniel L. Siegel, Oded Shoseyov
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Abstract
A method of releasing particulates from a solid matrix is provided. The method is effected adding to the solid matrix a degrading enzyme capable of degrading the solid matrix, to thereby release the particulates from the solid matrix.
Claims43
A method of releasing affinity adsorbed particulates from a solid matrix, the method comprising the step of adding to the solid matrix a degrading enzyme capable of degrading the solid matrix, to thereby release the affinity adsorbed particulates from the solid matrix, wherein said particulates are selected from the group consisting of viruses or portions thereof, cells or portions thereof and microorganisms or portions thereof, the method further comprising at least one step selected from the group consisting of eluting the particulates, recovering the particulates and separating the particulates from the solid matrix.
A method of releasing growth factor dependent cells from a polysaccharide matrix to which the growth factor dependent cells being adsorbed via an affinity complex including said growth factor and a polysaccharide binding peptide, the method comprising the step of adding to the polysaccharide matrix a polysaccharidase capable of degrading the polysaccharide matrix, to thereby release the growth factor dependent cells from the polysaccharide matrix.
A method of releasing a microorganism from a polysaccharide matrix to which the microorganism being adsorbed via an affinity complex including a polysaccharide binding peptide-receptor conjugate specific for the microorganism, the method comprising the step of adding to the polysaccharide matrix a polysaccharidase capable of degrading the polysaccharide matrix, to thereby release the microorganism from the polysaccharide matrix.
A method of releasing biological particulates from a solid matrix, the method comprising the step of adding to the solid matrix a degrading enzyme capable of degrading the solid matrix, to thereby release the biological particulates from the solid matrix, wherein the solid matrix includes a polysaccharide and further wherein said degrading enzyme is a polysaccharidase.
A method of releasing living particulates from a solid matrix, the method comprising the steps of:
- (a) adding to the solid matrix a degrading enzyme capable of degrading the solid matrix, to thereby release the living particulates from the solid matrix;
- and
- (b) recovering the living particulates.
A method of releasing affinity adsorbed particulates from a solid matrix, the method comprising the steps of:
- (a) adding to the solid matrix a degrading enzyme capable of degrading the solid matrix, to thereby release the affinity adsorbed particulates from the solid matrix;
- and
- (b) eluting the affinity adsorbed particulates.
Dependencies are inferred from the claim text.