Method for the fermentative production of D-pantothenic acid by enhancement of the panD gene in microorganisms
AssigneeDegussa-Huls AG
InventorsNicole Dusch, Jörn Kalinowski, Alfred Puhler
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Abstract
The invention is a method for the production of D-pantothenic acid by the fermentation of microorganisms in which at least the panD gene is enhanced (overexpressed), optionally in combination with the panB and/or panC gene, to provide for the enrichment of the pantothenic acid in the medium or the cells of the microorganisms.
Claims21
An isolated polynucleotide from Corynebacterium comprising a nucleotide sequence encoding the panD gene product, which is aspartate-1-decarboxylase, whose amino acid sequence is set forth in SEQ ID NO:
- 2.
An isolated polynucleotide from Corynebacterium comprising a nucleotide sequence that hybridizes to the antisense strand of SEQ ID NO:
- 1 and that encodes aspartate-1-decarboxylase.
A method for producing pantothenic acid comprising:
- a) transforming a vector into a microorganism to produce a recombinant microorganism, wherein said vector comprises a panD gene operably linked to a suitable regulatory sequence;
- b) growing said recombinant microorganism under conditions suitable for the production of pantothenic acid in an appropriate culture medium;
- and
- c) recovering pantothenic acid from said culture medium;
- wherein said panD gene encodes aspartate-1-decarboxylase and originates from Corynebacterium.
A method of producing pantothenic acid in Corynebacterium comprising:
- a) inserting an expression cassette upstream from the panD gene in said Corynebacterium;
- b) growing said Corynebacterium under conditions suitable for the production of pantothenic acid in an appropriate culture medium;
- and
- c) recovering pantothenic acid from said culture medium.
A method for producing pantothenic acid in Corynebacterium comprising:
- a) increasing the stability of the mRNA which is translated from the panD gene in said Corynebacterium;
- and/or
- b) preventing the degradation of the pcanD gene product in said Corynebacterium, which gene product is aspartate-1-decarboxylase;
- wherein said Corynebacterium is grown under conditions suitable for the production of pantothenic acid in an appropriate culture medium, and wherein pantothenic acid is recovered from said culture medium.
A method for producing pantothenic acid comprising:
- a) transforming a vector into a microorganism to produce a recombinant microorganism, wherein said vector comprises a panD gene, and wherein said vector further comprises a panB gene or a panC gene, which genes are all operably linked to a suitable regulatory sequence;
- b) growing said recombinant microorganism under conditions suitable for the production of pantothenic acid in an appropriate culture medium;
- and
- c) recovering pantothenic acid from said culture medium;
- wherein said panD gene encodes aspartate-1-decarboxylase, said panB gene encodes ketopantoate hydroxymethyltransferase and said panC gene encodes pantothenate synthetase and said panD, panB, and panC genes originate from Corynebacterium.
Dependencies are inferred from the claim text.