Polymerase enhancing factor (PEF) extracts PEF protein complexes isolated PEF proteins and methods for purifying and identifying same
AssigneeStratagene
InventorHolly Hogrefe
Life of this patent
Estimating the term…
Abstract
Novel extracts, proteins, and complexes are identified, purified, and analyzed, which improve the polymerization activity of nucleic acid polymerases. Included within the aspects of the invention are methods for identifying compositions with a polymerase enhancing activity, methods for purifying and using these compositions, and specific extracts, proteins, and complexes that function to enhance polymerase activity. Implications for numerous assays and techniques are described. For example, the invention can be used to enhance polymerase activity in a PCR process and to increase the sensitivity of a PCR-based assay.
Claims41
A non-naturally occurring composition of matter comprising at least one component possessing nucleic acid polymerase enhancing activity selected from the group consisting of:
- an isolated or purified naturally occurring polymerase enhancing protein obtained from an archeabacteria source;
- a wholly or partially synthetic protein having the same amino acid sequence as said naturally-occurring protein or analogs thereof possessing polymerase enhancing activity;
- polymerase-enhancing mixtures of one or more of said naturally occurring or wholly or partially synthetic proteins;
- polymerase-enhancing protein complexes of one or more of said naturally occurring or wholly or partially synthetic proteins;
- or polymerase-enhancing partially purified cell extracts containing one or more of said naturally occurring proteins.
An isolated or purified DNA having a sequence selected from the group consisting of:
- the sequence set forth in SEQ ID NO:
- 18, degenerate sequences thereof, or DNA sequences hybridizable therewith;
- the sequence set forth in SEQ ID NO:
- 42, degenerate sequences thereof, or DNA sequences capable of hybridizing therewith.
An antibody that binds to a protein having an amino acid sequence comprising one of SEQ ID NO:
- 19, 37-39, 41, 43-48.
Dependencies are inferred from the claim text.