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AssigneeStichting Institut voor Dierhouderij en Diergezonheld
InventorsDirk Franciscus Marinus van de Wiel, Petrus Antonius van Rijn, Robertus Jacobus Maria Moormann, Robert Hans Meloen
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The invention provides methods for the production of recombinant bovine Follicle Stimulating Hormone (bFSH) as well as vectors and cells for use in those methods. In particular the invention provides baculovirus based vectors which are capable of expression of bFSH in insect cells. bFSH is a heterodimeric protein belonging to a family of glycoprotein hormones which are produced in the pituitary or the placenta. It finds its use in many fertility related applications. Expression of bFSH in baculovirus/insect cell systems leads to a recombinant bFSH which has an unexpected high activity in a human FSH receptor assay and/or a bovine immature oocyte assay. The genes encoding the subunits of bFSH may be present on one baculovirus derived vector or on two or more vectors which are to be cotransfected.
A method for producing bovine follicle stimulating hormone, said method comprising:
A recombinant Baculovirus vector or a recombinant Baculovirus comprising at least a nucleic acid coding for the alpha subunit of bovine follicle stimulating hormone wherein said encoding nucleic acid comprises at least one ATTTA sequence having a destabilizing effect on mRNA, in a stretch of untranslated nucleotides at the nucleic acid's 3′ end.
A recombinant Baculovirus vector or a recombinant Baculovirus comprising at least a nucleic acid coding for the beta subunit of bovine follicle stimulating hormone wherein said encoding nucleic acid comprises at least one ATTTA sequence having a destabilizing effect on mRNA, in a stretch of untranslated nucleotides at the nucleic acid's 3′ end.
Dependencies are inferred from the claim text.