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US 6,183,978 B2Granted
Luciferase assay, compositions and kits for use in connection therewith
AssigneeTropix, Inc.
InventorsIrena Bronstein, Corinne E. M. Olesen, John C. Voyta, Yu-Xin Yan
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Abstract
An assay for the presence of luciferase in a biological sample offers heightened sensitivity, signal intensity and persistence. The assay is sensitive down to 50 fg luciferase. The biological sample is combined with essential ingredients luciferin, ADP, myokinase and Mg++. The myokinase converts ADP to ATP, necessary for the luciferase reaction, and AMP, which retards the reaction kinetics. The resulting assay exhibits a persistent glow emission which makes it adaptable to automation.
Claims10
A method of assaying for the presence of luciferase in a biological sample, comprising:
- combining said sample with a reaction composition which comprises luciferin, adenosine diphosphate (ADP) and myokinase;
- allowing said reaction composition to incubate with said biological sample;
- and
- inspecting said incubated biological sample and reaction composition to determine if light is emitted, wherein said light emission is indicative of the presence of luciferase in said biological sample and wherein the intensity of said light emission is a sustained glow that persists over an prolonged period of time to permit automated detection of the presence of luciferase in said sample.
1 more element
Dependencies are inferred from the claim text.