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AssigneeUMBI - University of Maryland Biotechnology Institute
InventorsAnita C. Wright, Jan L. Powell, J. Glenn Morris, Jr.
Estimating the term…
The present invention is directed to oligonucleotides used as amplification primers and assay probes for specific and sensitive for virulent strains of V. vulnificus. The target sequence of the probes and primers according to present invention is a capsular polysaccharide (CPS) transport gene (wza) of V. vulnificus. These probes can detect wza DNA or RNA in an unknown sample suspected to have pathogenic strains of V. vulnificus including human, animal, or environmental samples. The invention is also directed to in vitro-expressed protein from the cloned wza for production of polyclonal or monoclonal antibody that is specific for the wza gene product and will detect the V. vulnificus Wza protein in a sample comprising unknown protein.
A nucleotide sequence which hybridizes preferentially to the nucleic acid sequence shown in FIG. 1 (SEQ ID NO 1), or a fragment of said nucleic acid sequence, under appropriate hybridization conditions, said nucleotide sequence being about 20-50 bases in length and wherein hybridization of said nucleotide sequence is an indication of strains of V. vulnificus.
An isolated nucleotide sequence which hybridizes preferentially to the capsular polysaccaride transport gene from V. vulnificus, said nucleotide sequence being about 20-50 bases in length.
The nucleic acid sequence shown in FIG. 1 (SEQ ID NO 1) in isolated form.
Dependencies are inferred from the claim text.