Intron-based assay for detecting and characterizing chromosomal rearrangement
AssigneeRutgers, The State University of New Jersey
InventorAbram Gabriel
Life of this patent
Estimating the term…
Abstract
An assay is provided for detecting and characterizing chromosomal rearrangements in eukaryotic cells by genetically engineering a eukaryotic cell to contain a selectable/counter-selectable marker gene with a site for generating a double strand break within an intron of the eukaryotic cell; generating the double strand break; detecting cells which repair the double strand break by chromosomal rearrangements; isolating DNA from the detected cells; and characterizing the chromosomal rearrangement as a nonhomologous translocation, inversion, deletion or insertion.
Claims8
An assay for detecting and characterizing chromosomal rearrangements in eukaryotic cells comprising:
- (a) genetically engineering a eukaryotic cell to contain a selectable/counter-selectable marker gene with a site for generating a double strand break within an intron of the eukaryotic cell;
- (b) generating the double strand break;
- (c) detecting cells which repair the double strand break by chromosomal rearrangements;
- (d) isolating DNA from the detected cells;
- and
- (e) characterizing the chromosomal rearrangement as a nonhomologous translocation, inversion, deletion or insertion.
Dependencies are inferred from the claim text.