Back to results
US 6,183,959 B2Granted
Method for target site selection and discovery
AssigneeRibozyme Pharmaceuticals, Inc.
InventorJames D. Thompson
Life of this patent
Estimating the term…
Abstract
Nucleic acid catalysts, method of screening/selection for nucleic acid catalysts, synthesis of ribozyme libraries and discovery of gene sequences involved in a biological process are described.
Claims48
A method for identification of a nucleic acid molecule that modulates a process in a biological system comprising the steps of:
- a) introducing a random library of a nucleic acid catalyst into said biological system under conditions suitable for modulating said process, wherein said nucleic acid catalyst comprises a substrate binding domain and a catalytic domain, said substrate binding domain comprises a random sequence;
- and
- b) determining the nucleotide sequence of at least a portion of the substrate binding domain of said nucleic acid catalyst from said biological system in which the process has been modulated.
A method for identifying one or more nucleic acid molecules involved in a process in a biological system comprising the steps of:
- a) providing a library of a nucleic acid catalyst, with a substrate binding domain and a catalytic domain, wherein said substrate binding domain comprises a random sequence, to said biological system under conditions suitable for said process to be altered;
- b) identifying any said nucleic acid catalyst present in said biological system where said process has been altered;
- and
- c) determining the nucleotide sequence of at least a portion of the binding domain of said any said nucleic acid catalyst to allow said identification of said nucleic acid molecule involved in said process in said biological system.
1 more element
A method for identification of a nucleic acid catalyst that modulates a process in a biological system comprising the steps of:
- a) introducing a random library of a nucleic acid catalyst into said biological system under conditions suitable for modulating said process, wherein said nucleic acid catalyst comprises a substrate binding domain and a catalytic domain, said substrate binding domain comprises a random sequence;
- and
- b) identifying said nucleic acid catalyst from said biological system in which the process has been modulated.
Dependencies are inferred from the claim text.